Lidiya Petrova1, Hristina Kulina2, Anton Trifonov3, George Russev1, Kristiana Marinova4, Vanya Bogoeva1
Bulgarian Academy of Sciences, Institute of Molecular Biology, Sofia, Bulgaria
Plovdiv University, Faculty of Mathematics and Informatics, Plovdiv, Bulgaria
Sofia University, Faculty of Physics, Sofia, Bulgaria
Sofia University, Faculty of Biology, Sofia, Bulgaria
Correspondence to: Vanya Bogoeva E-mail: vanya.bogoeva@gmail.com
Abstract
Binding of porphyrins to biological macromolecules is important to be studied as an approach to keeping them in stable, effective and safe dosage forms. In this work we show the interaction of three porphyrin compounds: Hematoporphyrin IX, Mn- and Fe-porphyrins with plant lectin Wheat germ agglutinin (WGA). Porphyrin binding to WGA was monitored by the changes in the intrinsic protein fluorescence. Conformational changes due to the formation of WGA-porphyrin complexes, were shown by the hyperbolic binding curves. The calculated dissociation constants KD (0.16 μМ to 0.24 µМ) indicate high affinity of WGA for the three porphyrins. Most probably they occupy the same high affinity sites. In conclusion, since WGA binds cancer cells and interacts with antitumor compounds, it could be useful as a drug delivery molecule.
Keywords porphyrins, wheat germ agglutinin, protein