SELECTION AND MASS PRODUCTION OF SANTALUM ALBUM L. CALLI FOR INDUCTION OF SESQUITERPENES
Julien Crovadore1, Michel Schalk2, François Lefort2
University of Applied Sciences of Western Switzerland, Geneva Institute of Technology, Architecture and Landscape, Research Institute Earth Nature and Environment, Jussy, Switzerland
Firmenich SA, Biotechnology Department, Corporate R&D Division, Geneva, Switzerland
Correspondence to: François Lefort E-mail: francois.lefort@hesge.ch
Abstract
Young hypocotyl segments obtained from aseptically germinated seeds of Santalum album L. (5 weeks old) were used to induce callus formation. Growth of different calli types was induced on hypocotyls segments under different conditions. Resulting green calli were maintained on MS medium and Gamborg basal medium supplemented with 2,4D (2,4-Dichlorophenoxyacetic acid) (0.5 µM) and Kin (Kinetin) (10 µM). Numerous concentrations of 2,4D and Kin were tested in combination with inducers of the sesquiterpene metabolism and one promising callus type was selected for mass production. This interesting type of callus has been used for searching functional genes in the biosynthetic pathway leading to sesquiterpenes molecules.
Keywords in vitro culture, sandalwood oil, Santalum album, sesquiterpene
