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Biotechnology & Biotechnological Equipment 27 (6), 4263 - 4268 (2013)
http://dx.doi.org/10.5504/BBEQ.2013.0096

AGRICULTURE AND ENVIRONMENTAL BIOTECHNOLOGY

DECOLORIZATION OF INDUSTRIAL DYES BY IMMOBILIZED MYCELIA OF TRAMETES VERSICOLOR

Albert Krastanov1, Ralitza Koleva1, Zlatka Alexieva2, Ivanka Stoilova1

  1. University of Food Technologies, Department of Biotechnology, Plovdiv, Bulgaria
  2. Bulgarian Academy of Science, Department of Microbiology, Sofia, Bulgaria

Correspondence to: Ralitza Koleva E-mail: koleva.ralica@gmail.com

Abstract

The aim of this study was to compare the decolorization of several of the most utilized synthetic dyes in textile applications by immobilized white-rot fungus Trametes versicolor. Different immobilization methods for achieving maximum decolorization were explored. The initial dye concentration was 125 mg/L and the immobilized preparation concentration was 10 % (w/v). Different degrees of decolorization were observed. Different operational stability of the immobilized preparations was accomplished. Increase of the immobilized preparation concentration up to 30 % (w/v) was investigated. The study was performed in two stages: simulated incubation in a ‘batch’ reactor and trickle-bed continuous flow reactor. During the decolorization process, laccase activity was detected. The dye-decolorizing activity of the immobilized culture was found to be associated with the processes of biodegradation, bio-oxidation and biosorption.

Keywords
decolorization, immobilization, laccase, synthetic dyes, Trametes versicolor

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