Article

Biotechnology & Biotechnological Equipment 27 (6), 4236 - 4242 (2013)
http://dx.doi.org/10.5504/BBEQ.2013.0088

AGRICULTURE AND ENVIRONMENTAL BIOTECHNOLOGY

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PERSICARIA AMPHIBIA, A SERIOUS TERRESTRIAL WEED IN NORTHERN GREECE: A COMBINED MOLECULAR AND MORPHOLOGICAL APPROACH TO IDENTIFICATION AND TAXONOMY

Thomas K. Gitsopoulos¹, Ioannis Vasilakoglou², Georgios Tsoktouridis³

Hellenic Agricultural Organization “Demeter”, Plant Protection Institute of Thessaloniki, NAGREF, Thessaloniki, Greece
Technological Educational Institute of Thessaly, Department of Agricultural Technology, Larissa, Greece
Hellenic Agricultural Organization “Demeter”, Laboratory for the Conservation and Evaluation of Native and Floricultural Species, Thessaloniki, Greece

Correspondence to: Georgios Tsoktouridis E-mail: gtsok1@yahoo.co.uk

Published online 07 November 2013; Published 18 December 2013

Abstract

The versatile rhizomatous terrestrial form of the perennial weed Persicaria amphibia is reported to cause severe infestations in potato crops, which are mainly cultivated as a monoculture in the area of Kato Nevrokopi located at the Greek–Bulgarian boundaries. This weed cannot be easily eradicated from potato crops in Northern Greece and therefore, the accurate taxonomic identification of P. amphibia has a high economical importance. The generic classification of P. amphibia has been unstable, mostly due to different morphological interpretations with other Persicaria species. Molecular studies based on DNA sequence data from the rbcL and matK plastid regions supported 100 % homology with P. amphibia. Further analysis using the psbK-psbI, atpF-atpH, rpoC1 and rpoB plastid regions identified distinct differences with other Persicaria species suggesting the application of psbK-psbI and atpF-atpH as potential molecular markers for identification and phylogenetic analysis of Persicaria taxa. Morphology description and ecology characteristics concerning the terrestrial form of P. amphibia are provided.

A multidisciplinary approach with both molecular and morphological information is described in this study and provides potential useful tools for the identification and taxonomy of Persicaria species.

Keywords
potato crop, molecular identification, morphology, classification, chloroplast DNA, Polygonaceae

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Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., et al. (1997) Nucleic Acids Res, 25, 3389-3402. doi:10.1093/nar/25.17.3389
Brandbyge J. (1993) In: The Families and Genera of Vascular Plants. II. Flowering Plants (Dicotyledons): Magnoliid, Hamamelid and Caryophyllid families (K. Kubitzki, J.G. Rohwer, V. Bittrich, Eds.), Springer-Verlag, Berlin, 531-544.
Burke J.M., Sanchez A., Kron K., Luckow M. (2010) Am. J. Bot., 97, 1377-1390. doi: 10.3732/ajb.1000022
CBOL Plant Working Group (2009) P. Natl. Acad. Sci. USA, 106, 12794-12797.
Chase M.W., Cowan R.S., Hollingsworth P.M., van den Berg C., et al. (2007) Taxon, 56, 295-299.
Decraene L.-P.R., Akeroyd J.R. (1988) Bot. J. Linn. Soc., 98, 321-371. doi: 10.1111/j.1095-8339.1988.tb01706.x
Devey D.S., Chase M.W., Clarkson J.J. (2009) Taxon, 58, 7-15.
Ekman S., Knutsson, T. (1994) Nord. J. Bot., 14, 23-25 doi: 10.1111/j.1756-1051.1994.tb00564.x
Fassett N.C. (1949) Brittonia, 6, 369-393.
Fazekas A.J., Burgess K.S., Kesanakurti P.R., Graham S.W., et al. (2008) PLoS ONE, 3, e2802. doi:10.1371/journal.pone.0002802
Ford C.S., Ayres K.L., Haider N., Toomey N., et al. (2009) Bot. J. Linn. Soc., 159, 1-11. doi: 10.1111/j.1095-8339.2008.00938.x
Galasso G., Banfi E., Mattia F.d., Grassi F., et al. (2009) Atti della Societ à Italiana di Scienze Naturali e del Museo Civico di Storia Naturale di Milano, 150, 113-148.
Greene E.L. (1904) Leaflets of Botanical Observation and Criticism, Washington DC, pp. 17-32.
Grime, J.P., Hodgson, J.G., Hunt R. (1988) Comparative Plant Ecology: a Functional Approach to Communities of British Species, Unwin Hyman Publ., London.
Hanf M. (1983) The Arable Weeds of Europe, with their Seedlings and Seeds, BASF United Kingdom, p. 400.
Haraldson K. (1978) Symbolae Botanicae Upsalienses, 22.
Hilu K.W., Black C., Diouf D, Burleigh J.G. (2008) Mol. Phylogenet. Evol., 48, 1120-1130. http://dx.doi.org/10.1016/j.ympev.2008.05.021
Hollingsworth P.M., Graham S.W., Little D.P. (2011) PLoS ONE, 6, e19254. doi:10.1371/journal.pone.0019254
Jones J. (2001) Laboratory Guide for Conducting Soil Tests and Plant Analysis, CRC Press, Boca Raton FL.
Kent D.H. (1992) List of Vascular Plants of the British Isles, Botanical Society of the British Isles, London.
Kim J.S., Hong J.-K., Chase M.W., Fay M.F., et al. (2013) Bot. J. Linn. Soc., 172, 5-21. doi: 10.1111/boj.12039
Kim S.-T., Donoghue M.J. (2008). Syst. Bot., 33, 77-86. http://dx.doi.org/10.1600/036364408783887302
Kim S.-T., Sultan S.E., Donoghue M.J. (2008) P. Natl. Acad. Sci. USA, 105, 12370-12375. www.pnas.org_cgi_doi_10.1073_pnas.0805141105
Kress W.J., Erickson D.L. (2007) PLoS ONE, 2, e508. doi:10.1371/journal.pone.0000508.g001
Kress W.J., Wurdack K.J., Zimmer E.A., Weigt L.A., et al. (2005) P. Natl. Acad. Sci. USA, 102, 8369-8374. www.pnas.org_cgi_doi_10.1073_pnas.0503123102
Kwak M., Kim M.-H., Won H, Park C.-W. (2006) Korean Journal of Plant Taxonomy, 36, 21-39.
Lahaye R., van der Bank M., Bogarin D., Warner J., et al. (2008) In: Proceedings of the National Academy of Sciences of USA, 105, 2923-2928. www.pnas.org_cgi_doi_10.1073_pnas.0709936105
Li A.J., Li A.-R., Bojian B., Grabovskaya-Borodina A.E., et al. (2003) Polygonaceae. Flora of China 5. Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, pp. 277-350
Li D.Z., Gao L.M., Li H.T., Wang H., et al. (2011) P. Natl. Acad. Sci. USA, 108, 19641-19646. www.pnas.org/cgi/doi/10.1073/pnas.1104551108
Lousley J.E., Kent D.E. (1981) Docks and Knotweeds of British Isles, Botanical Society of the Bitish Isles, London.
Mitchell R.S., Dean J.K. (1978) N. Y. State Mus. Bull., 431, 1-79.
Mitchell R.S. (1968) University of California Publications in Botany, 45, 1-54.
Newmaster, S.G., Ragupathy, S., Janovec J. (2009) Int. J. Comp. Appl. Tech., 35, 50-60. doi: 10.1504/IJCAT.2009.024595
Olmstead R.G., Palmer J.D. (1994) Am. J. Bot., 81, 1205-1224.
Partridge W.J. (2001) J. Ecol., 89, 487-501. doi: 10.1046/j.1365-2745.2001.00571.x
Rai I.D., Singh G., Rawat G.S. (2013) Phytotaxa, 92, 13-19. http://dx.doi.org/10.11646/phytotaxa.92.1.2
Rodwell J.S. (1995) British Plant Communities, Vol. 4, Aquatic Communities, Swamps and Tall-Herb Fens, Cambridge University Press, Cambridge.
Sanchez I., Kron K.A. (2008) Syst. Bot., 33, 87-96. http://dx.doi.org/10.1600/036364408783887456
Sculthorpe C.D. (1967) The Biology of Aquatic Vascular Plants, Edward Arnold, London.
Silvertown J., McConway K., Gowing D., Dodd M., et al. (2006) P. Roy. Soc. B-Biol. Sci., 273, 39-44.
Stace C.A. (1997) New Flora of the British Isles, 2nd Ed., Cambridge University Press, Cambridge.
Thompson J.D., Higgins D.G., Gibson T.J. (1994) Nucleic Acids Res., 22, 4673-4680. doi:10.1093/nar/22.22.4673
Van der Weide R.Y., Bleeker P., Riemens M., Bastiaans L. (2008) In: EWRS-NJF Workshop, 10-12 November 2008, Wageningen, p. 5.
Zhao D.-P., Wang K.-M., Hou Y.-T. (2012) Bulletin of Botanical Research, 32, 77-83.

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PERSICARIA AMPHIBIA, A SERIOUS TERRESTRIAL WEED IN NORTHERN GREECE: A COMBINED MOLECULAR AND MORPHOLOGICAL APPROACH TO IDENTIFICATION AND TAXONOMY

Thomas K. Gitsopoulos¹, Ioannis Vasilakoglou², Georgios Tsoktouridis³

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Article

PERSICARIA AMPHIBIA, A SERIOUS TERRESTRIAL WEED IN NORTHERN GREECE: A COMBINED MOLECULAR AND MORPHOLOGICAL APPROACH TO IDENTIFICATION AND TAXONOMY

Thomas K. Gitsopoulos1, Ioannis Vasilakoglou2, Georgios Tsoktouridis3

Abstract

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Thomas K. Gitsopoulos¹, Ioannis Vasilakoglou², Georgios Tsoktouridis³