Article

The aim of this study was to investigate the proliferation and osteogenic differentiation of mesenchymal stem cells in contact with porous coated titanium implant. Human mesenchymal stem cells (BM-MSCs) were obtained from bone marrow aspirate from the femoral canal during elective hip replacement. The MSCs were isolated and cultured. The resulting cells were seeded on porous coated titanium plates obtained from prosthesis blanks that had all the characteristics of the final product. The same cells were grown on classic plastic polystyrene plates as a control specimens. The proliferation and osteogenic differentiation of the isolated and cultured mesenchymal stem cells were examined. Our results show that under the same conditions there were no significant differences between the study and control groups. Titanium implants with porous coated surface provide favorable conditions for the proliferation and differentiation of bone marrow stem cells and did not inhibit their development. In conclusion, it may be possible to advance this methodology to stimulate healing of bone defects and particularly massive osteolysis in the revision arthroplasty setting. MSCs may provide an alternative to the use of autologous and allogenic bone-grafts in orthopedic surgery.