Biotechnology & Biotechnological Equipment 27 (6), 4317 - 4322 (2013)http://dx.doi.org/10.5504/BBEQ.2013.0010
PHARMACEUTICAL BIOTECHNOLOGY
EXCELLENT STABILITY AND SYNTHETIC ACTIVITY OF LIPASE FROM B. STEAROTHERMOPHILUS MC7 IMMOBILIZED ON TIN DIOXIDE IN ENVIRONMENTALLY FRIENDLY MEDIUM
Maya Guncheva1 , Momchil Dimitrov1 , Margarita Kambourova2 Bulgarian Academy of Sciences, Institute of Organic Chemistry with Centre of Phytochemistry, Sofia, Bulgaria Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria Correspondence to: Maya Guncheva E-mail: maiag@orgchm.bas.bg
Published 18 December 2013
Abstract A thermostable lipase from B. strearothermophilus MC7 was stabilized via immobilization on nanostructured tin dioxide (nanoSnO2 -MC7). The synthetic activity of nanoSnO2 -MC7 in two ionic liquids containing chloride anions was assessed. In the presence of 3-methyl-1-octyl imidazolium chloride, the stearyl stearate production proceeded at a much higher reaction rate than in solvent-free medium and 95 % conversion of the substrates was achieved for 5 h at 65 °C. Our results showed that it also had a beneficial effect on the operational and thermal stability of the immobilized lipase MC7. In this green solvent, the biocatalyst was almost fully active after eight synthetic cycles and up to 60 % of the initial activity of nanoSnO2 -MC7 lipase was preserved after long heating at 75 ºC.
Keywords Bacillus stearothermophilus MC7 lipase, immobilization, wax-ester synthesis, ionic liquids, solvent-free system
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